The genome of the cell forms a loosely-defined 'nucleoid', shown here in yellow, and interacts with many DNA-binding proteins, shown in tan and orange. The characteristic two-membrane cell wall of gram-negative bacteria is shown in green, with many lipopolysaccharide chains extending from the surface and a network of cross-linked peptidoglycan strands between the membranes. This painting shows a cross-section through an Escherichia coli cell.

Diversity, Equity, Inclusion, and AccessĪcknowledgement: Illustration by David S.

Exploring the Structural Biology of Bioenergy.Exploring the Structural Biology of Cancer.Perform multi-color SMLM experiments on the two sCMOS cameras.

Acquire 4-color images without the need for mechanical filter change – making your multi-color experiments even faster.Image any possible color combination with the two cameras, and with minimal signal crosstalk as enabled by the integrated multi-bandpass emission filters.Increase the productivity of your fixed cell experiments by doubling the amount of information gained during the same amount of time.Acquire a super-resolved real-time snapshot of an entire living cell by picking a low exposure time.Perform true simultaneous two-color imaging within your entire field of view – without any delays within the image such as can occur when using scanning-based technologies or during consecutive data acquisition of different channels.Equip your Elyra 7 with a Duolink adapter to operate two sCMOS cameras and use all the advantages that a widefield-based technology can offer: Simultaneous imaging of the involved structures is key to proper understanding of these highly dynamic processes. Investigation of living samples very often focuses on interactions of different proteins or organelles. Thus, you can acquire 3D data from a whole cell with consistent molecular precision. The PSF is reshaped for encoding the Z position so while acquiring only one plane, you get volume information of 1.4 µm depth at 50 – 80 nm axial resolution. In addition, Elyra 7 provides you with 3D SMLM mode based on PRILM technology. The ZEN software will seamlessly perform the image reconstruction of your data. With Elyra 7 you can use SMLM techniques such as PALM, dSTORM and PAINT to achieve lateral resolution of 20 – 30 nm. The localizations are plotted in a new image to create the super-resolution image. Once recorded, the molecule is turned to its off-state and the cycle of activation/deactivation is repeated until all molecules are captured. This lets you determine its center of mass with a localization precision that far exceeds the extension of the PSF. In single-molecule localization microscopy (SMLM), fluorescent molecules are sparsely activated so that only one out of many will be in its on-state within a single point spread function (PSF). ZEISS Nanoscale and Correlative Analysis.

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